Fig. S10.

Colocalization of recombinant hGBP1 and marker proteins around latex beads. Unstimulated HeLa cells were cotransfected with mCherry-hGBP1 as well as GFP-tagged marker proteins and incubated with 2 µm latex beads for 4 h to specify distinct stages of the phagocytic pathway. (A) GFP-Rab5. (B) GFP-Rab7. (C) GFP-Rab9. (D) LAMP1-GFP. (Scale bar, 5 µm.) Arrows mark engulfed latex beads within the cytoplasm. (E) Life-cell imaging of recruitment of hGBP1 and Rab5 to latex bead phagosomes. Uninduced HeLa cells transfected with mCherry-hGBP1 and GFP-Rab5 were incubated with blue fluorescent latex beads for 4 h and uptake of beads was followed by life-cell imaging. Arrows indicate a phagocytosed bead, which is attached to Rab⁺ (green) and hGBP1⁺ (red) punctate structures. See also Movie S6. Time scale, hours:minutes:seconds (h:min:s). (Scale bar, 10 µm.)