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Morpholino mediated knock down and CRISPR/Cas9 mediated knock out of NvNkx2.5 in Nematostella. (A) A splice blocking anti-sense morpholino oligo was designed targeting the splice site between intron1 and exon2. (B) PCR showing an amplified DNA fragment of 880bp from cDNA from control embryos but absent in cDNA from NvNkx2.5 MO injected embryos. NvNkx2.5 gene deletion efficiency was determined by amplifying the NvNkx2.5 gene fragment.
