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Molecular cloning of dnc-1. dnc-1 maps approximately +4.8 map units, on LGIV (left of dpy-20). dnc-1 mutants were rescued by cosmid ZK593. A single allele of dnc-1 has a lesion in the predicted gene ZK593.5, as shown below exon 12. We identified dnc-1(or404ts) in a temperature-sensitive screen for embryonic lethal mutants described in Encalada et al. (2000).
