Figure 3.
Depletion of spindle checkpoint proteins restores normal mitotic timing in one-cell mutant embryos with cytoskeletal abnormalities and delayed mitoses. Bars represent the duration of mitosis from prometaphase (PNEBD) to the onset of anaphase (A) or PNEBD to NER (B), in one-cell embryos. Numbers inside boxes indicate the duration of each mitotic stage in seconds. Mean durations are plotted with SEM error bars. Table 2 shows Student's t test statistical comparisons of mitotic timing between wild-type and mutant embryos. The p values for mitotic timing comparisons between apo-5 and the following double mutants at the p ≤ 0.05 level are shown in parentheses: apo-5; mdf-1(0.042); apo-5; mdf-2 (0.0082); apo-5; Cebub-1 (0.17); apo-5; wrm-1 (0.47); apo-5; hcp-1/2 (0.013); apo-5; CeMCAK (0.032); and apo-5; CeCENP-C (0.00067).