Fig. S2.

R258C-SM α-actin does not retard cell migration in cells from a second patient. Representative images for control (C2-T) and ACTA2-R258C (P2-T) cells pretreated for 48 h with/without Ad-MRTF-A followed by indicated assay. (A) Phase contrast images for scratch assay are from start of assay (0 h) and after 24 h. Epifluorescence images of nuclei stained with Hoechst at 24 h are shown. Location of initial scratch margins is indicated by dashed white lines. (Scale bars, 100 μm.) (B) Images of Transwell membranes with cells stained by crystal violet. Membranes were fixed 6 h after seeding cells.