Fig. 3.
Loss of Dmrta2 in cortical NPCs accelerate neurogenesis in vitro. (A) Day 4 and day 6 cultures were immunostained for Nestin, revealing comparable generation of NPCs in the control and Dmrta2−/− cultures. (B) qPCR analysis of neuronal differentiation markers. Data are representative of three independent differentiation experiments. (C) Immunostaining and quantification of cells expressing an immature neuronal marker, Tubb3. Two-way ANOVA identified a significant increase in the overall production of Tubb3+ neurons by Dmrta2−/− NPCs (F1,16 = 8.005; P = 0.012). (D) Immunostaining and quantification of cells expressing mature neuronal marker NeuN. Two-way ANOVA revealed a significant increase in the overall maturation of neurons derived from Dmrta2−/− NPCs (F1,16 = 11.991; P = 0.003). (Scale bars: 100 µm.)