Fig. 6.
Functional analysis of FDT > A and D205 > A NK92-MI transduced cell lines. NK92-MI cell lines transduced with CD16A WT, FDT > A, or D205 > A mutants were functionally tested in ADCC and redirected lysis experiments. NK cells were cultivated alone or in combination with Raji or P815 cell lines loaded, or not, with αCD20 (Rituximab) and CD16-specific mAb (3G8), respectively. After 2-h coculture, CD107a staining was measured by flow cytometry. Data represent mean of three experiments, and statistical significance was calculated using one-way ANOVA-Dunnett posttest for multiple comparison (**P < 0.01; ***P < 0.001; n.s., not statistically significant P > 0.05).