Fig. S5.

A single isoleucine residue in the mouse CD247 TM sequence that prevents assembly with CD16A is located in the core TM packing interface. (A) Alignment of human and mouse CD247 TM sequence highlighting the position of the variation identified in ref. 16 as blocking association with CD16A (magenta). (B) A view down the long axis of the human CD247-CD16A trimer model showing the internal position of L¹⁶ in the F²⁰²D²⁰⁵T²⁰⁶ interface. We hypothesize that substitution of leucine with the β-branched side-chain of isoleucine is not well accommodated in the tightly packed interface, leading to defective assembly.