Figure 2.

Expression of the XM chimera blocks the translocation of annexin 2 but does not modify the actin depolymerization required for exocytosis. Chromaffin cells infected with pSFV1EGFP-subX or pSFV1EGFP-XM were maintained under resting conditions or stimulated for 5 min with 10 μM nicotine and then processed for immunocytochemistry. Cells were double labeled with anti-p36 antibodies and rhodamine-conjugated phalloidin. Images were recorded in the same optical section by a triple exposure procedure. Bars, 10 μm. The star indicates a noninfected cell. (B) The histogram represents a semiquantitative analysis of the amount of fluorescent phalloidin detected in resting and nicotine-stimulated cells (±SEM, n = 13). Actin was found to depolymerize similarly in infected cells in which p36 translocation was impaired in comparison with noninfected cells that had p36 in the periphery.