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. 2017 Mar 24;3(2):18. doi: 10.3390/ncrna3020018

Figure 2.

Figure 2

miRNA qPCR with pooled synthetic oligo RNAs: “synthetic cell lines.” To determine the specificity of both Exiqon LNA miRCuRY PCR and ThermoFisher Taqman miRNA qPCR, we combined the four isomiRs of hsa-miR-21-5p (Table 1) such that we formed four pools in which each one of the isomiRs in turn was present at five times the concentration of the other three. We then performed one RT and three qPCR replicates with each technique. Panel (a) shows the results for LNA qPCR, while panel (b) shows the results for Taqman qPCR. Colored bars show the Ct value for each of the synthetic pools, while grey bars show the expected Ct value for each pool. Three stars (***) indicate a p-value ≤ 0.001, while two stars (**) indicate a p-value ≤ 0.01.