Table 2.
Primers For the mouse pri-miR-9-2 gene locus*
| Region | Primer sequence | Product Size | |
|---|---|---|---|
| pre-mir-9-2 | Forward | CCTTGTGAGGGAAGCGAGTT | |
| coding exon | Reverse | CGTTCCTCGGTGACCTTGAA | 112 |
| Forward | ATTTCCCAAACCCCTCTAGATCC | ||
| 1 | Reverse | TGGAGACATGCATGATAATGGGA | 60 |
| Forward | CTTCACTCTGCACTCCGAGG | ||
| 2 | Reverse | GGGAGAGAAAAGGGCGCTAA | 101 |
| Forward | ACATACTGGGGGTCGTCGAA | ||
| 3 | Reverse | GGCTGTAGTTGTAGCCTGCG | 86 |
| Forward | TGACCTCGCAGCTAAACCTT | ||
| 4 | Reverse | ACATGCTTTCGGGCTCCAC | 81 |
| Forward | AGCCACCCAAGTCCAGAGAA | ||
| 5 | Reverse | AGGGAAACAGCGACACCTAC | 98 |
*
For primer mapping to the pre-miR-9-2 gene, see Figure 4.