Figure 6. Nlrp3 deficiency reverses part of the phenotype of DC-ABC^DKO mice.

Abca1^fl/flAbcg1^fl/flNlrp3^+/−, Abca1^fl/flAbcg1^fl/flNlrp3^−/− (referred to as controls), DC-ABC^DKONlrp3^+/−, and DC-ABC^DKONlrp3^−/− mice were 10 weeks old. (A–B). LNs were isolated. Representative pictures are shown (A) and LNs were weighed (B). n=11–25. Splenic CD11b⁺ cells were isolated and IL-1β secretion (o/n) was assessed (C). n=5–10. Splenic cell homogenates were stained with an antibodies to CD4, fixed and permeabilized, and stained for T_bet, or its isotype control. CD4⁺T_bet⁺ T-cells were assessed and values were corrected for their respective isotype controls (D–E). n=5–8. Data in B, C, E are presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001, by one-way ANOVA with Bonferroni post-test.