Figure 2.

XGef and CPEB interact directly in vitro. Bacterially expressed GST-XGef (or GST, as a control) was immobilized on glutathione-Sepharose beads that were then incubated with bacterially expressed, purified His-CPEB. The input CPEB protein (INPUT), bead bound fractions (Beads) and postbinding supernatant fractions (Supernatant) were analyzed by SDS-PAGE and immunoblotting with anti-CPEB antibodies (IB, top). Roughly equivalent amounts of GST and GST-XGef were present on the glutathione beads (bottom, Coomassie stained, CS, gel).