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. 2005 Mar;16(3):1152–1164. doi: 10.1091/mbc.E04-07-0585

Figure 3.

Figure 3.

XGef is present in an mRNP-containing CPEB protein and c-mos mRNA in both prophase I oocytes and early maturing oocytes. Protein complexes were immunoprecipitated from prophase I oocytes (lanes 2–6) and from oocytes that had been incubated in progesterone for 2.5 h (lanes 7 and 8). Antibodies used for immunoprecipitation included ns-IgG, anti-XGef and anti-CPEB. Immune complexes were analyzed by immunoblotting with anti-CPEB antibody (IP/IB: CPEB, top). RNA extracted from these complexes was analyzed by RT-PCR for the presence of c-mos mRNA (middle two panels) and β-actin mRNA (bottom). Lanes 5 and 6 represent reamplification of samples shown in lanes 4 and 3, respectively. Lanes 7 and 8 represent reamplification of ns IgG and anti-XGef immunoprecipitations from maturing oocytes where the primary amplification product was too low to visualize. Both c-mos mRNA and β-actin mRNA were successfully amplified from total ovary RNA (lane 1).