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. 2005 Mar;16(3):1152–1164. doi: 10.1091/mbc.E04-07-0585

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Copyright © 2005, The American Society for Cell Biology

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Figure 6. — Interaction between XGef and CPEB is necessary for the influence of XGef overexpression on oocyte maturation. (A) Effect of HA-XGef, HA-Globin, HA-XGef(65–360), and HA-XGef(Δ65–234) overexpression on progesterone-induced oocyte maturation, monitored by appearance of GVBD. Inset, anti-HA antibody immunoblot analysis of HA-XGef, HA-Globin, HA-XGef(65–360), and XGef(Δ65–234) levels in oocytes. (B) Histogram of the percentage of GVBD in oocytes overexpressing HA-XGef or HA-XGef(65–360) when Globin-overexpressing oocytes within the same experiment had reached 50% GVBD. Results from 10 independent experiments with at least 30 oocytes per injection were statistically analyzed using the Student's t test. ^*P < 0.01.