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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Eur J Immunol. 2016 Oct;46(10):2454–2466. doi: 10.1002/eji.201546274

Fig. 1. Clodronate-loaded liposomes selectively deplete CD11c+CD11b+ DCs or iDCs.

Fig. 1

Naïve C57BL/6 mice were i.p. injected with PBS-loaded or clodronate-loaded liposomes, splenocytes were harvested at 24 hours later and analyzed with flow cytometry. (A) Percentages of DCs (CD11c+) among splenocytes (left). Results were statistically analyzed and shown as mean ± SEM (n=3 each group) (right). (B) CD11c+ cells were then gated and percentages of CD11b+ and CD8+ cells were analyzed (left). Results were statistically analyzed and shown as mean ± SEM (n=3 each group) (right). (C) Expression of CD11c vs. CD83, CD80, and CD86 in splenocytes (left). Results were statistically analyzed and shown as mean ± SEM (n=3 each group) (right). (D) Expression of CD83, CD80, and CD86 in total CD11c+ cells and in CD11b+, CD8+, CD11b−CD8− cells of gated CD11c+ cells. *p<0.05, ***p<0.001, unpaired Student’s t test. One representative of 3 experiments is shown.