Fig. 2. Clodronate-loaded liposome partly block i.v. MOG-induced EAE suppression.

Mice were immunized with MOG_35–55 in CFA to induce EAE. MOG_35–55 or PBS was i.v. injected at days 0, 3, and 6 p.i. Clodronate- or PBS-loaded liposome was i.p. injected 24 hours before and 14 days p.i. (A) Clinical signs were scored daily following a 0–5 scale. Data were pooled from three independent experiments and presented as mean clinical score ± SEM (n=11–15 each group). (B) On day 21 p.i., spinal cords were harvested and 5-µm sections were stained with H&E and LFB. Demyelination areas are designated by arrows. (Magnifications, × 10). (C) Inflammation and demyelination were scored using a 0–3 scale and expressed as mean scores ± SEM (n=3 each group). *p<0.05, ***p<0.001, two-way ANOVA test. One representative of 3 experiments is shown.