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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Eur J Immunol. 2016 Oct;46(10):2454–2466. doi: 10.1002/eji.201546274

Fig. 3. I.v. tolerance induced increase of CD11c+CD11b+ DCs is abrogated by injection of clodronate-loaded liposomes.

Fig. 3

Mice immunized for EAE induction were i.v. injected with MOG or PBS, while PBS-loaded or clodronate-loaded liposomes were i.p. injected. Splenocytes were isolated 21 days p.i. and analyzed by flow cytometry. (A) FACS profile of macrophages (F4/80+ cells) in total splenocytes (left). Results were statistically analyzed and shown as mean ± SEM (n=3 each group) (right). (B) M1 (IA+) and M2 (CD206+) macrophages in gated F4/80+ cells (left). Results were statistically analyzed and shown as mean ± SEM (n=3 each group) (right). (C) DCs (CD11c+ cells) in total splenocytes (left). Results were statistically analyzed and shown as mean ± SEM (n=3 each group) (right). (D) CD11c+CD11b+ DCs (CD11b+ cells in gated CD11c+ cells) and CD11c+CD8+ DCs (CD8+ cells in gated CD11c+ cells) (left). Results were statistically analyzed and data shown as mean ± SEM (n=3 each group). *p<0.05, ** p<0.01, ***p<0.001, two-way ANOVA test. One representative of 3 experiments is shown.