Table 1.
Dominant ts effect of a G→E change in the third P-loop of yeast DHC-1
| Yeast strain | Plasmid | Temp. (°C) | % Cells with >1 nucleusa | nb |
|---|---|---|---|---|
| Wild type | 30 | 0.2 | 403 | |
| 16 | 0 | 298 | ||
| Wild type | dhc1(G→E)c | 30 | 17.4 | 339 |
| 16 | 3.2 | 219 | ||
| Wild type | DHC1d | 30 | 0 | 328 |
| 16 | 0.8 | 380 | ||
| dhc1Δ | 30 | 17.3 | 347 | |
| 16 | 28.2 | 298 | ||
| dhc1Δ | dhc1(G→E)c | 30 | 23.2 | 387 |
| 16 | 31.2 | 269 | ||
| dhc1Δ | DHC1d | 30 | 2.1 | 334 |
| 16 | 4.0 | 373 |
Transformed or untransformed haploid cells were grown for 2—4 in YPD medium after selection on URA- plates. Statistical analysis of the data is in Supplemental Table s1.
a
Yeast cells were fixed and stained with DAPI. Dividing cells (i.e. cells with buds) were scored for the presence of more than one nucleus
b
Total number of dividing cells
c
Plasmid containing full-length dhc1 with a mutation that causes Gly2421 to be replaced by Glu
d
Plasmid containing full-length wild-type DHC-1