Figure 1.

H₂O₂ induced apoptosis of cultured CGCs.

(A) Representative images of CGCs incubated in medium containing vehicle control or 50 μM H₂O₂, for the indicated times. Neurons were stained with Hoechst 33258 to visualize condensed nuclei Scale bar: 10 μm. (B) Apoptotic rates in CGCs were quantified by scoring the percentage of neurons with pyknotic nuclei. The data are expressed as the mean ± SEM (n = 3, analysis of variance and Student-Newman-Keuls post hoc test). *P < 0.05, vs. control. CGCs: Cerebellar granule cells; h: hours.