Figure 3.

1-Butanol suppresses AVP-induced myotube formation. The cells were cultured for 9 d in 1% BSA medium, in the absence (a) or presence (b–d) of AVP, with the addition of 0.5% 1-butanol (c) or 2-butanol (d). Sarcomeric myosin immunofluorescence was then examined, by using a specific mAb and a rhodamine-conjugated secondary antibody, to assess the formation of terminally differentiated myotubes. The nuclei were labeled by DAPI staining. No signs of toxicity were apparent in the presence of alcohols. Bar, 20 μm.