Table 2. Effect of zerumbone on microRNA expressions in differentiated 3T3-L1 cells and C57BL/6N mice.
| HF | HF + Z | D | D+Z | |
|---|---|---|---|---|
| mmu-miR-146b | 3.39 ± 0.03 | 1.84 ± 0.02* | 8.12 ± 0.85 | 3.43 ± 0.06# |
| mmu-miR-297b | 3.18 ± 0.01 | 1.23 ± 0.01* | 0.58 ± 0.01 | 1.28 ± 0.03# |
| mmu-miR-34a | 3.14 ± 0.02 | 2.58 ± 0.06* | 6.93 ± 0.34 | 1.90 ± 0.04# |
| mmu-miR-469 | 2.87 ± 0.03 | 1.46 ± 0.15* | 0.46 ± 0.26 | 3.35 ± 0.11# |
| mmu-miR-139-3p | 2.63 ± 0.03 | 1.20 ± 0.05* | 0.03 ± 0.0 | 0.54 ± 0.02# |
| mmu-miR-21 | 2.50 ± 0.21 | 2.24 ± 0.2 | 6.17 ± 0.25 | 1.06 ± 0.06# |
| mmu-miR-466E | 2.40 ± 0.01 | 0.98 ± 0.01* | 0.57 ± 0.01 | 1.07 ± 0.04# |
| mmu-miR-22* | 2.18 ± 0.01 | 1.68 ± 0.20* | 0.35 ± 0.02 | 0.53 ± 0.00# |
| mmu-miR-324 | 2.08 ± 0.01 | 1.53 ± 0.01* | 0.32 ± 0.01 | 0.51 ± 0.03# |
| mmu-miR-143 | 1.93 ± 0.03 | 1.53 ± 0.07* | 1.27 ± 0.02 | 0.95 ± 0.01# |
| mmu-miR-720 | 1.59 ± 0.03 | 1.29 ± 0.05* | ||
| mmu-miR-15b | 1.46 ± 0.04 | 0.56 ± 0.03* | ||
| mmu-miR-302a | 1.42 ± 0.01 | 0.92 ± 0.03* | ||
| mmu-miR-466h | 1.34 ± 0.04 | 1.48 ± 0.06* | ||
| mmu-miR-197 | 1.31 ± 0.01 | 1.09 ± 0.05* | ||
| mmu-miR-423 | 1.27 ± 0.05 | 0.96 ± 0.05* | ||
| mmu-miR-715 | 1.25 ± 0.01 | 1.31 ± 0.16* | ||
| mmu-miR-467 | 1.24 ± 0.00 | 1.63 ± 0.1* | ||
| mmu-miR-709 | 0.97 ± 0.01 | 0.73 ± 0.03* | ||
| mmu-miR-122 | 0.69 ± 0.04 | 0.05 ± 0.06* | ||
| mmu-miR-802 | 0.37 ± 0.01 | 2.76 ± 0.10* |
Results are the means ± SD. For epididymal adipose tissue, expressions of miRNAs are shown as fold-change relative to the level of NC group. *P < 0.05 versus HF group. For 3T3-cells, expressions of miRNAs are shown as fold-change relative to the level of non-differentiated preadipocytes. #P < 0.05 versus D group.
HF, high-fat diet-fed mice; HF+Z, high-fat diet containing 0.025% zerumbone-fed mice; D, differentiated adipocytes; D+Z, differentiated adipocytes treated with10 μM zerumbone.