Skip to main content
. 2017 Apr 8;8(23):37394–37408. doi: 10.18632/oncotarget.16962

Figure 5. LSS-11 inhibits viability of colorectal cancer cells through apoptosis and cell cycle arrest.

Figure 5

(A) Colony formation ability of SW480 cells treated with indicated concentrations of LSS-11 for 14 days. **p < 0.01; ***p < 0.0001. (B) Confocal microscopic observation of SW480 cells treated with 2 μM of LSS-11 for 24 h, F-actin and nucleus were stained by TRITC-phalloidin and Hoechst33258, respectively. Disruption of cytoskeleton and cell shrinkage are indicated by arrows, while nuclear fragmentation and chromatin condensation are indicated by triangles. (C) and (D) Flow cytometric analyses of SW480 cells treated with 0.5 μM LSS-11 for 72 h to show sub-G1 apoptotic cells (C) and cell cycle distribution (D). Please note the × axis in (C) is exponential and × axis in (D) is linear. (E) and (F) Cells were treated with different concentrations of LSS-11 for indicated time, then harvested and blotted by specified antibodies, GAPDH was blotted as an internal control to assure equal loading.