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Upper panel: Contiguous sections showing control tumor (A) vasculature based on CD31 immunohistochemistry, (B) tumor perfusion based on Hoechst 33342 distribution, and (C) hypoxia based on immunohistochemistry for perfused pimonidazole. Lower panel: (D-F) corresponding whole mount sections from tumor 24 hours after single dose of 12 Gy. Magnified sections (white box in D) are shown in (G, H) and (I).
