Table 1. Primary antibodies.
Antigen | Source | Dilution | Supplier |
---|---|---|---|
CD133 | Mouse | 1:200 (ICC) | Abcam |
Sox2 | Rabbit | 1:200 (ICC) | Santa Cruz |
1:1000 (WB) | |||
Nestin | Rabbit | 1:200 (ICC/IHC) | Millipore |
MBP | Goat | 1:200 (ICC/IHC) | Santa Cruz |
1:1000 (WB) | |||
GFAP | Rabbit | 1:400 (ICC/IHC/WB) | Boster |
Olig1 | Rabbit | 1:1000 (WB) | Millipore |
Olig2 | Rabbit | 1:400 (IHC) | Millipore |
1:1000 (WB) | |||
PCNA | Mouse | 1:200 (IHC) | Dako |
β-catenin | Rabbit | 1:1000 (WB) | Beyotime |
P-β-catenin | Mouse | 1:1000 (WB) | Santa Cruz |
GSK-3β | Rabbit | 1:1000 (WB) | Beyotime |
pGSK-β | Rabbit | 1:1000 (WB) | Beyotime |
β-actin | Mouse | 1:1000 (WB) | Santa Cruz |