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. 2017 Apr 19;8(23):37568–37583. doi: 10.18632/oncotarget.17247

Figure 3. Effects of CPZ on CcO expressing COX4-1 or COX4-2 isoform.

Figure 3

(A) The effect of CPZ on CcO activity was tested in mitochondrial extracts from cells with CcO expressing COX4-1 (TgCOX4-1) or COX4-2 (TgCOX4-2). Graphs represent the level at which CcO activity is inhibited in the presence of PBS (control) or CPZ (up to 50 μM). The results are averages from triplicate determinations from two independent experiments. (B) Effect of CPZ on the proliferation of cells with CcO expressing COX4-1 or COX4-2. (C) Bioenergetic profile of TgCOX4-1 intact cells in the presence of PBS (control) or CPZ (5 μM). Oxygen consumption was measured using sequential injection of oligomycin (2 μg/ml), FCCP (1 μM), and antimycin A (2.5 μM). (D) Basal respiration, ATP-linked respiration and reserve respiratory capacity are shown. Results represent the means ± SD of 3 independent experiments. (E) and (F) In vitro limiting dilution assays and quantification of GSCs from cells with CcO expressing COX4-1 and treated with CPZ at the concentrations indicated. Results are the average from two independent experiments. *p < 0.05; **p < 0.01; and ***p < 0.001.