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(A) Smad3 expression from VSMCs and TAD patients. (B) miR-145 expression from VSMCs and TAD patients. Western blot and RT-PCR detection of Smad3 expression from VSMCs after miR-145 mimics (C) and miR-145 inhibitor (D). (E) Dual luciferase reporter assay of WT and Mut Smad 3’ UTR.*P < 0.05, *P < 0.01, ***P <0.001 vs. Control or Normal group, n=6 in each group.
