Figure 1.

Expression of the Chlamydomonas KAP gene in response to deflagellation. (A) A Southern blot of wild-type (R) and S1D2 (S) genomic DNA was hybridized with the ∼3.4-kb insert from the KAP cDNA clone CL70g01. A single EcoRI/XhoI restriction fragment was seen in wild-type, and a polymorphic fragment was seen in S1D2. (B) A Northern blot of total RNA isolated from wild-type cells before (0) and 45 min after deflagellation (45) was hybridized with the KAP cDNA (top panel). Expression of the ∼3.4-kb KAP transcript was enhanced by deflagellation, as compared with a probe (Cry1) for a ribosomal protein subunit used as a loading control (bottom panel).