Figure 4.

Identification of a KAP mutation in fla3-1. (A) Sequence analysis of the KAP gene in fla3-1 revealed a point mutation at nucleotide 6853 in exon 17 that changes amino acid residue 753 from a phenylalanine to a leucine. (B) Diagram of the KAP polypeptide showing the position of fla3-1 mutation relative to the armadillo repeats. (C) The fla3-1 mutation does not alter the level of the KAP subunit in whole cells. Western blots of wild-type, fla3, and fla10 cells grown at 21°C or overnight at 33°C were probed with antibodies against KAP, FLA10, and tubulin. Each lane was loaded with extracts prepared from ∼5 × 10⁶ cells. The relative intensities of the KAP and FLA10 signals were normalized to the tubulin signals to control for small variations in protein loading. No significant changes in the levels of the KAP subunit were observed in the fla3-1 mutant at either the permissive or restrictive temperature.