Table 1.
Effects of the fla3 mutation on flagellar length
| Strain | Average length (μm) | Average length excluding zero length flagella (μm) | % Aflagellate cells |
|---|---|---|---|
| Vegetative cells | |||
| Wild-type (137c) | 11.1 ± 3.5 (105) | 11.6 ± 2.6 (100) | 4.8 |
| fla3 | 8.0 ± 4.2 (107) | 8.9 ± 3.5 (97) | 9.3 |
| fla3::KAP-GFP | 10.7 ± 3.1 (102) | 11.1 ± 2.3 (98) | 3.9 |
| Gametic cells | |||
| Wild-type (137c) | 12.6 ± 3.8 (107) | 13.2 ± 2.5 (102) | 4.7 |
| fla3 | 9.4 ± 3.7 (85) | 10.6 ± 1.3 (75) | 11.8 |
Flagellar length measurements are expressed as mean ± SD (sample size). Cells were grown with aeration in TAP medium (vegetative growth) or transferred to M-N/5 for 4 h to induce gametogenesis. All measurements were made at 21°C. The mean flagellar lengths of fla3 cells are significantly shorter (p < 0.001) than those of wild-type cells for both vegetative and gametic cells. Rescue of fla3 cells with the epitope-tagged KAP restores flagellar lengths to wild-type levels.