Table 2.

Velocity and frequency of particles undergoing anterograde and retrograde IFT

Strain	Anterograde frequency (p/s)	Retrograde frequency (p/s)	Frequency ratio	Total frequency (p/s)	Anterograde velocity (μ/s)	Retrograde velocity (μ/s)
Wild-type (21gr)	1.3 ± 0.5 (21)	2.2 ± 0.6 (21)	0.6 ± 0.1 (21)	3.7 ± 1.0 (21)	1.7 ± 0.3 (416)	2.8 ± 0.6 (360)
Wild-type (137c)	1.3 ± 0.5 (11)	2.2 ± 0.6 (11)	0.6 ± 0.1 (11)	3.5 ± 1.0 (11)	1.8 ± 0.3 (176)	2.6 ± 0.7 (180)
fla3	0.5 ± 0.2 (22)	1.9 ± 0.5 (22)	0.2 ± 0.1 (22)	2.3 ± 0.6 (22)	1.6 ± 0.3 (377)	2.8 ± 0.5 (420)
fla3::KAP-GFP	1.5 ± 0.4 (13)	2.8 ± 0.6 (13)	0.6 ± 0.1 (13)	4.2 ± 0.8 (13)	2.2 ± 0.3 (220)	3.4 ± 0.7 (259)

All measurements are expressed as mean ± SD (sample size). For frequency measurements, n = total number of cells, and for velocity measurements, n = total number of particles. Frequency is expressed as particles per second (p/s), and velocity is expressed as microns per second (μ/s). The frequency ratio is the frequency of anterograde particles divided by the frequency of retrograde particles for each cell. Because the fla3 mutation was originally isolated in the 137c background, but backcrossed into 21gr, we analyzed particle movement in both strains. No significant differences in particle frequency or velocity were observed between the two wild-type strains. For fla3 cells, the frequency of anterograde particles is significantly reduced, but anterograde particle velocity is only slightly decreased relative to wild-type cells. No statistically significant differences (p < 0.001) were observed for retrograde particle frequency or velocity. Transformation of fla3 with the GFP-tagged KAP construct (fla3::KAP-GFP) restores the frequency of anterograde particles (and the frequency ratio) to wild-type levels.