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. 2015 Oct 29;1:15009. doi: 10.1038/npjamd.2015.9

Figure 3.

Figure 3

Genomic recombination and gene expression in adipocyte-specific Bdnf/Ntrk2 deletion models created using Adipoq-Cre mice. (a) PCR analysis of genomic DNA isolated from various tissues of Adipoq-TrkB CKO mice and from epididymal white adipose tissue (EWAT) of their littermate controls and wild-type mice. Littermate controls were homozygous for the floxed Ntrk2 allele, but did not carry Cre recombinase. (b) Real-time PCR analysis of Ntrk2 expression in various tissues of Adipoq-TrkB CKO mice and their littermate controls (Control); n=5. **P<0.01, ***P<0.001. (c) PCR analysis of genomic DNA isolated from various tissues of Adipoq-BDNF CKO mice and from epididymal white adipose tissue (EWAT) of their littermate controls and wild-type mice. Littermate controls were homozygous for the floxed Bdnf allele, but did not carry Cre recombinase. (d) Real-time PCR analysis of Bdnf expression in various tissues of Adipoq-BDNF CKO mice and their littermate controls (Control); n=5. Data are shown as the mean±s.e.m. CKO, conditional knockout.