Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 24;2:16029. doi: 10.1038/npjamd.2016.29

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

PMC Copyright notice

Gene expression and differentiation induction in senesced cells. (a) CD105 expression in 520d-5p/NHDF cells subjected to irradiation was confirmed 75 days after transfection of 520d-5p using immunocytochemistry (ICC; top). 520d-5p/NHDF cells just prior to apoptosis (77 days post-transfection) underwent differentiation induced by differentiation agents to confirm by ICC whether they had mesenchymal stem cell-like properties. Representative images show that CD105 was expressed during the senesced state although irradiated NHDF expressed CD105 strongly in proliferative status. (b) Osteocalcin was expressed in irradiated NHDF cells after differentiation induction, but the expression of FABP and aggrecan was not observed (bottom). Left, unstained; middle, rhodamine-conjugated antibody; right, second antibody alone.