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. 2016 Nov 24;2:16029. doi: 10.1038/npjamd.2016.29

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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(a) A luciferase reporter expression assay was performed to examine whether hsa-miR-520d-5p targets ATM. Out of the more than nine predicted binding sites in the 3ʹ-UTRs of ATM, two core sequences (5ʹ-UUCUUUGUA-3ʹ and 5ʹ-UUUUUUGUA-3ʹ in the 3ʹ-UTR of ATM) were chosen to test for hsa-miR-520d-5p binding. These sites were predicted based on the two databases described above. (b) A luciferase reporter expression assay revealed that hsa-miR-520d-5p bound to the 3ʹ-UTR of ATM, resulting in a reduction of luciferase expression. In contrast, a mismatch of hsa-miR-520d-5p (standardization control) and a scramble group did not significantly bind to the 3ʹ-UTR sites.