Figure 7.

Predicted Klar isoforms. The diagram shows the likely extent of the three Klar protein isoforms α, β, and γ (klar gene structure as in Figure 1). Isoforms carry C-terminal targeting domains: KASH (α and γ) or LD (β). Isoform α (predicted to be 250 kDa) can partially rescue the klar nuclear migration defect in photoreceptors. Isoform β is associated with lipid droplets and is expressed from the proximal promoter. It seems likely that it contains most, if not all exons from 2 to 15X because it is affected by point mutations in exons 3, 11, 12, and 14 and is recognized by the exon 9-specific Klar-M antibody, and because cDNA LD08331 contains all exons from 6 to 15X. If so, its molecular weight is 202 kDa. Isoform γ (62 kDa) is present perinuclearly in ovaries and likely contains exons G, 16, 17, and 18 (based on perinuclear ovary staining in class I alleles and the sequence of cDNA GH05539).