Table 1.
HIV testing strategy and algorithms per study site.
| Conakry, Guinea | Kitgum, Uganda | Arua, Uganda | Homa Bay Kenya | Douala, Cameroun | Baraka, DRC | Baraka*, DRC | Kitgum*, Uganda | |
|---|---|---|---|---|---|---|---|---|
| Testing strategy |
Serial |
Serial with tie-breaker |
Serial with tie-breaker |
Serial with tie-breaker |
Serial |
Serial |
Parallel with confirmatory testing |
Parallel with confirmatory testing |
| Determine HIV-1/2 (Alere, USA) |
Determine HIV-1/2 (Alere, USA) |
Determine HIV-1/2 (Alere, USA) |
Determine HIV-1/2 (Alere, USA) |
Determine HIV-1/2 (Alere, USA) |
Determine HIV-1/2 (Alere, USA) |
Determine HIV-1/2 (Alere, USA) |
Determine HIV-1/2 (Alere, USA) |
|
| Testing algorithm | ImmunoFlow HIV-1–HIV-2 (Core Diagnostics, UK) |
Uni-Gold HIV(Trinity Biotech, Ireland) |
HIV-1/2 Stat-Pak (Chembio, USA) |
Uni-Gold HIV (Trinity Biotech, Ireland) |
ImmunoComb II HIV-1&2 BiSpot (Orgenics/Alere, Israel) |
Uni-Gold HIV (Trinity Biotech, Ireland) |
Uni-Gold HIV (Trinity Biotech, Ireland) |
Uni-Gold HIV (Trinity Biotech, Ireland) |
| |
If discordant: retest in 6 weeks |
If discordant: HIV 1/2Stat-Pak (Chembio, USA) |
If discordant: Uni-Gold HIV (Trinity Biotech, Ireland) |
If discordant: GS HIV-1/HIV-2 PLUS O EIA (Bio-Rad, USA) at CDC, Kisumu, Kenya |
If discordant: retest in 6 weeks |
If discordant: retest in 6 weeks |
On all double positives ImmunoComb II HIV 1&2 CombFirm (Orgenics/Alere, Israel) |
On all double positives: ImmunoComb II HIV 1&2 CombFirm (Orgenics/Alere, Israel) |
| Sample type | EDTA plasma | EDTA plasma | Capillary whole blood | Capillary whole blood | EDTA whole blood | Capillary whole blood | EDTA plasma | EDTA plasma |
DRC: Democratic Republic of Congo.
*Algorithm using a simple confirmatory assay (ImmunoComb II HIV 1&2 CombFirm, Orgenics, Israel) with an alternative interpretation by Médecins sans Frontières.