Table 5.
Detailed testing results of discordant rapid test results per study site.
| Study site | No. (%) Discordant samples | Sample IDs | Test result on site | HIV status on site | Test results reference laboratory | HIV status by reference laboratory |
|---|---|---|---|---|---|---|
| Conakry | 4 (0.9) | 151, 153, 198, 327 | Determine: reactive ImmunoFlow: non-reactive |
Inconclusive | ELISA: non-reactive | HIV negative |
| Kitgum | 10 (2.3) | 82, 617 | Determine: non-reactive Uni-Gold: reactive Stat-Pak: non-reactive |
HIV negative | ELISA: non-reactive | HIV negative |
| 114, 123 | Determine: reactive Uni-Gold: non-reactive Stat-Pak: non-reactive |
HIV negative | ELISA: non-reactive | HIV negative | ||
| 138, 519, 559 | Determine: reactive Uni-Gold: non-reactive Stat-Pak: non-reactive |
HIV negative | ELISA: reactive LIA: non-reactive (sgp120: −, gp41: −, p31: −, p24: −, p17: −, gp105: −, gp36: −) |
HIV negative | ||
| 148 | Determine: reactive Uni-Gold: non-reactive Stat-Pak: reactive ImmunoComb Combfirm: positive (p24+, p31−, gp120+, gp41+, gp36−) |
HIV positive | ELISA: reactive LIA: reactive (sgp120: 3+, gp41: 3+, p31: 2+, p24: 4+, p17: 3+, sgp105: −, gp36) |
HIV-1 positive | ||
| 196 | Determine: reactive Uni-Gold: non-reactive Stat-Pak: non-reactive |
HIV negative | ELISA: reactive LIA: reactive (sgp120: −, gp41: 1+, p31: −, p24: 1+, p17: 2+, sgp105: −, gp36: −) |
HIV-1 positive | ||
| 205 | Determine: reactive Uni-Gold: non-reactive Stat-Pak: reactive ImmunoComb Combfirm: positive (p24+, p31−, gp120+, gp41+, gp36+) |
HIV positive | ELISA: reactive LIA: reactive (sgp120: −, gp41: 2+, p31: −, p24: 2+, p17: − sgp105: −, gp36) |
HIV-1 positive | ||
| Arua | 12 (2.7) | 251, 344, 354, 387, 402, 431, 440, 441, 465, 489, 495 | Determine: reactive Stat-Pak: non-reactive Uni-Gold: non-reactive |
HIV negative | ELISA: non-reactive | HIV negative |
| 620 | Determine: reactive Stat-Pak: non-reactive Uni-Gold: reactive |
HIV positive | ELISA: reactive LIA: reactive (sgp120: 3+, gp41: 3+, p31: 3+, p24: 2+, p17: 3+, gp105: −, gp36: −) |
HIV-1 positive | ||
| Homa-Bay | 9 (1.8) | 5 | Determine: reactive Uni-Gold: non-reactive EIA GS: reactive |
HIV positive | ELISA: non-reactive | HIV negative |
| 66, 138, 184, 298, 422 | Determine: reactive Uni-Gold: non-reactive EIA GS: non-reactive |
HIV negative | ELISA: non-reactive | HIV negative | ||
| 170 | Determine: reactive Uni-Gold: non-reactive EIA GS: reactive |
HIV positive | ELISA: reactive LIA: reactive spg120: 2+, gp41: 3+, p31: 3+, p24: 4+, p17: 3+, spg105: −, gp36: −) |
HIV-1 positive | ||
| 177 | Determine: reactive Uni-Gold: non-reactive EIA GS: reactive |
HIV positive | ELISA: reactive LIA: reactive (spg120: 2+, gp41: 2+, p31: 2+, p24: 4+, p17: 3+, spg105: −, gp36: −) |
HIV-1 positive | ||
| 449 | Determine: reactive Uni-Gold: non-reactive EIA GS: reactive |
HIV positive | ELISA: reactive LIA: reactive (spg120: −, gp41: 2+, p31: −, p24: 2+, p17: +, spg105: −, gp36: −) |
HIV-1 positive | ||
| Douala | 10 (2.2) | 006, 011, 165, 202, 363, 374, 378, 397, 398, 457 | Determine: reactive BiSpot: non-reactive |
Inconclusive | ELISA: non-reactive | HIV negative |
| Baraka | 54 (10.9) | 24, 28, 56, 68, 83, 84, 85, 96, 98, 104, 105, 120, 128, 139, 140, 147, 160, 161, 174, 184, 191, 196, 197, 203, 228, 231, 239, 260, 277, 296, 300, 318, 320, 328, 334, 341, 364, 367, 368, 370, 376, 389, 396, 408, 409, 420, 429, 443, 444, 471, 472, 474 | Determine: reactive Uni-Gold: non-reactive |
Inconclusive | ELISA: non-reactive | HIV negative |
| 86 | Determine: non-reactive Uni-Gold: reactive |
Inconclusive | ELISA: reactive LIA: reactive (sgp120: − gp41: +/−, p31: −, p24: −, p17: −, sgp105: −, gp36: −) |
HIV-1 positive | ||
| 476 | Determine: non-reactive Uni-Gold: reactive ImmunoComb Combfirm: positive (p24+, p31−, gp120+, gp41+, gp36−) |
Inconclusive | ELISA: reactive LIA: reactive (sgp120: − gp41: +/−, p31: −, p24: 2+ p17: 1+, sgp105: −, gp36: −) |
Inconclusive (no DBS for PCR) |
Determine: Determine HIV-1/2 (Alere, USA); ImmunoFlow: ImmunoFlow HIV 1–HIV 2 (Core Diagnostics, UK); Uni-Gold: Uni-Gold HIV (Trinity Biotech, Ireland); Stat-Pak: HIV 1/2 Stat-Pak (Chembio, USA); ImmunoComb CombFirm: ImmunoComb II HIV 1&2 CombFirm (Orgenics, Alere, Israel); EIA GS: GS HIV-1/HIV-2 PLUS O EIA (Bio-Rad, USA); BiSpot: ImmunoComb II HIV 1&2 BiSpot (Orgenics, Alere, Israel); DBS: dried blood spot.