Table 2.
Impact of mutant expression on keratin filament organization in transfected cells
| DNA construct transfected
|
||||||
|---|---|---|---|---|---|---|
| wtK5 | K5E477K | K5D328H | K5-1649delG | K5CΔ111 | K5CΔ41 | |
| Transfection efficiency (%) | 16.9 | 25.9 | 21.5 | 24.7 | 25.7 | 17.8 |
| Abnormal cells (%) | 6.2 | 25.5 | 11.4 | 16.6 | 17.9 | 14.0 |
| Severe (%) | 5.6 | 19.6 | 7.6 | 8.7 | 13.1 | 7.4 |
| Mild (%) | 0.6 | 5.9 | 3.8 | 7.9 | 4.8 | 6.6 |
For each DNA construct tested, >1000 cells were included as part of this analysis. Mildly abnormal refers to transfected cells in which the transfected protein exhibited an abnormal distribution but the endogenous keratin IF network seemed normal. Severely abnormal refers to transfected cells in which both the transfected protein and the endogenous keratin IF network exhibited a grossly abnormal distribution.