CHO2.46 cells are defective in the surface expression of GPI-anchored proteins. (A) Cell viabilities against two microbial toxins, proaerolysin (1) and C. septicum α-toxin (2), assessed by MTT assay. Circles, wild-type CHO K1; triangles, parental CHO F21; diamonds, mutant CHO2.46. (B) FACS analysis showing the cell surface expression of GPI-anchored proteins CD59, and DAF. 1, CHO K1 cells, which do not express CD59 and DAF, used as a negative control; 2, parental CHO F21; and 3, mutant CHO2.46.