Figure 5.

(A) Alignment of amino acid sequences of human, rat, and mouse PIG-X, and S. cerevisiae Pbn1p. Asterisks, amino acids conserved in all four proteins; single dots, positions in which similar residues are conserved; double dots, positions in which highly similar residues are conserved; leucine residues (L) at the translation initiation site are in boldface type. N-terminal sequence of mature rat PIG-X are boxed. Signal peptide in rat PIG-X and putative transmembrane domains are underlined and bold-underlined, respectively. (B) Hydropathy plot of rat PIG-X according to the Kyte–Doolittle method (Kyte and Doolittle, 1982).