Figure 6.

PIG-X associates with and stabilizes PIG-M. (A) CHO K1 cells were cotransfected with two cDNAs, followed by affinity precipitation with either anti-FLAG- or glutathione-beads. From the supernatant of affinity precipitation, unbound proteins were recovered by using other affinity beads as indicated. The corresponding amount of samples were applied onto SDS-PAGE and detected by Western blotting with either anti-FLAG or anti-GST antibodies. Lanes 1 and 4, empty plasmid only; lanes 2 and 5, FLAG-tagged PIG-X and GST-tagged PIG-M; lanes 3 and 6, FLAG-tagged ALDH and GST-tagged PIG-M. (B) CHO2.46 cells were first cotransfected with HSV-tagged-PIG-M and -DPM3 (as a control). The transfectant cells were further transfected transiently with PIG-X (lane 1) or an empty vector (lane 2). Expression levels of PIG-M and DPM3 were assessed by immunoprecipitation and Western blotting with anti-HSV.