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. Author manuscript; available in PMC: 2017 Jul 18.
Published in final edited form as: J Am Chem Soc. 2016 Feb 8;138(6):1800–1803. doi: 10.1021/jacs.5b12648

Figure 3.

Figure 3

Photosensitized cross-linking of H-Ras on membranes. (A) Fluorescence intensity fluctuation of a high-density His6-Ras sample (~1600/μm2) in an underlabeling experiment. Initially, the sample is fully labeled (magenta trace). Then, the sample is washed and underlabeled (green trace). (B) Autocorrelation of intensity fluctuations. (C) Diffusion coefficients of fully labeled His6-Ras samples with (blue) and without imaging buffer (magenta) measured by FCS. (D) Step-size distributions of high density Ras (~4500/μm2) under the various imaging conditions.