Figure 3.

Long-term treatment with ceramide causes growth arrest and apoptosis. HeLa cells treated with 2.5 μM D-e-Cer or Et were subjected to FACS analysis at 24, 36, and 48 h (A), annexin-V-rhodamine staining and microscopic analysis at 36 h (B), Western blot analysis of PARP cleavage (C), and Western blot analysis of caspase 9 and 3 activation of (D) at 36 h. p85, the 85-kDa cleaved fragment of PARP; Cas. 9, caspase 9; and Cas. 3, caspase 3. This figure is representative of at least three independent experiments.