Figure 8.

Brefeldin A causes the similar effects to ceramide treatment. (A) HeLa cells were treated with dimethyl sulfoxide (DMSO) or 100 nM BFA for 8 h and were photographed under the inverted microscopy. These cells were fixed and co-stained with the anti-giantin (green) and phalloidin-rhodamine (red) (B) or stained with the anti-paxillin antibody (C) before confocal microscopy. HeLa cells treated with DMSO or BFA for various times were subjected to FACS analysis (D). G1, G1 phase, G2, G2 phase, S, S phase; A, apoptosis. This figure is representative of at least three independent experiments.