Figure 5. Upstream regulatory analysis of significantly differentially expressed proteins and verification by Western blot.

Of all of the candidate transcription factors, MYC, E2F1 and CCND1 occupied the highest proportion of the upstream regions of the differentially expressed proteins (A). Enriched KEGG pathway analysis on DEPs regulated by E2F1 is presented as a bar chart (B). Western blot analysis with E2F1 antibody was performed. Data are shown as mean±SD from three densitometry evaluation; ^aP<0.05; ^bP<0.005. β-actin was used for internal control (C).