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Molecular Biology of the Cell logoLink to Molecular Biology of the Cell
. 2005 Mar;16(3):1568.
PMCID: PMC551518

The printer would like to correct an error in the article “Loss of Function of KRE5 Suppresses Temperature Sensitivity of Mutants Lacking Mitochondrial Anionic Lipids” (Mol. Biol. Cell [2005] 16, 665–675). All instances of “[ρ]” should have been removed from the column titled “Medium” in Table 2. The correct Table 2 is published below:

Table 2.

Cell wall composition in pgs1Δ and suppressor mutants

Alkaline-insoluble glucan
Alkaline-soluble
Strain Medium β-1,6 β-1,6 + β-1,3 β-1,3 β-1,3-glucan Chitin
WT YPD 37.8 ± 0.9 141.4 ± 4.4 103.6 100% 4.65 ± 0.39
WT ρ0 YPD 23.7 ± 1.4 114.7 ± 8.1 91.0 50 ± 3% 4.54 ± 0.26
pgs1Δ ρ0 YPD 12.1 ± 1.9 74.7 ± 1.4 62.6 38 ± 2% 12.36 ± 1.67
pgs1Δ kre5W1166X ρ0 YPD 7.7 ± 2.5 155.8 ± 1.4 148.1 174 ± 15% 12.35 ± 0.39
pgs1Δ ρ0 YPDS 21.0 ± 1.6 98.8 ± 18.8 77.8 90 ± 25% 8.09 ± 1.52
pgs1Δ ρ0 + PGS1 Ura- 25.8 ± 5.3 131.6 ± 33.5 105.8 100% 7.76 ± 0.32
pgs1Δ ρ0 + vec Ura- 21.4 ± 2.1 69.6 ± 22.7 48.2 41 ± 6% 12.04 ± 0.80
pgs1Δ kre5W1166X ρ0 + vec Ura- 7.4 ± 1.8 118.4 ± 17.3 111.0 151 ± 21% 12.09 ± 3.14
pgs1Δ kre5W1166X ρ0 + KRE5 Ura- 27.1 ± 0.9 99.3 ± 9.6 72.2 52 ± 5% 10.52 ± 0.37

Glucan and chitin levels were measured as described in Materials and Methods in wild-type (FGY3), ρ0, pgs1Δ (QZY24B), and suppressor mutant pgs1Δ kre5W1166X (QZY11A) cells grown in YPD or YPDS; pgs1Δ (QZY24B) cells transformed with empty vector pYES2/CT (+vec) or pYES2/CT-PGS1 (+PGS1); and pgs1Δ kre5W1166X suppressor mutant (QZY11A) cells transformed with empty vector YCp50 (+vec) or the genomic clone of KRE5 (+KRE5) grown in synthetic ura- medium. Alkaline insoluble glucan and chitin are expressed as micrograms per milligram of cell dry weight. Alkaline soluble β-1,3-glucan in cells grown in complex medium (top) was expressed as a percentage of that of wild-type (FGY3) cells. Alkaline soluble β-1,3-glucan in cells grown in synthetic ura- medium (bottom) was expressed relative to pgs1Δ (QZY24B) cells transformed with pYES2/CT-PGS1. Data represent three independent experiments.


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