Table 2.
Cell wall composition in pgs1Δ and suppressor mutants
| Alkaline-insoluble glucan
|
Alkaline-soluble
|
|||||
|---|---|---|---|---|---|---|
| Strain | Medium | β-1,6 | β-1,6 + β-1,3 | β-1,3 | β-1,3-glucan | Chitin |
| WT | YPD | 37.8 ± 0.9 | 141.4 ± 4.4 | 103.6 | 100% | 4.65 ± 0.39 |
| WT ρ0 | YPD | 23.7 ± 1.4 | 114.7 ± 8.1 | 91.0 | 50 ± 3% | 4.54 ± 0.26 |
| pgs1Δ ρ0 | YPD | 12.1 ± 1.9 | 74.7 ± 1.4 | 62.6 | 38 ± 2% | 12.36 ± 1.67 |
| pgs1Δ kre5W1166X ρ0 | YPD | 7.7 ± 2.5 | 155.8 ± 1.4 | 148.1 | 174 ± 15% | 12.35 ± 0.39 |
| pgs1Δ ρ0 | YPDS | 21.0 ± 1.6 | 98.8 ± 18.8 | 77.8 | 90 ± 25% | 8.09 ± 1.52 |
| pgs1Δ ρ0 + PGS1 | Ura- | 25.8 ± 5.3 | 131.6 ± 33.5 | 105.8 | 100% | 7.76 ± 0.32 |
| pgs1Δ ρ0 + vec | Ura- | 21.4 ± 2.1 | 69.6 ± 22.7 | 48.2 | 41 ± 6% | 12.04 ± 0.80 |
| pgs1Δ kre5W1166X ρ0 + vec | Ura- | 7.4 ± 1.8 | 118.4 ± 17.3 | 111.0 | 151 ± 21% | 12.09 ± 3.14 |
| pgs1Δ kre5W1166X ρ0 + KRE5 | Ura- | 27.1 ± 0.9 | 99.3 ± 9.6 | 72.2 | 52 ± 5% | 10.52 ± 0.37 |
Glucan and chitin levels were measured as described in Materials and Methods in wild-type (FGY3), ρ0, pgs1Δ (QZY24B), and suppressor mutant pgs1Δ kre5W1166X (QZY11A) cells grown in YPD or YPDS; pgs1Δ (QZY24B) cells transformed with empty vector pYES2/CT (+vec) or pYES2/CT-PGS1 (+PGS1); and pgs1Δ kre5W1166X suppressor mutant (QZY11A) cells transformed with empty vector YCp50 (+vec) or the genomic clone of KRE5 (+KRE5) grown in synthetic ura- medium. Alkaline insoluble glucan and chitin are expressed as micrograms per milligram of cell dry weight. Alkaline soluble β-1,3-glucan in cells grown in complex medium (top) was expressed as a percentage of that of wild-type (FGY3) cells. Alkaline soluble β-1,3-glucan in cells grown in synthetic ura- medium (bottom) was expressed relative to pgs1Δ (QZY24B) cells transformed with pYES2/CT-PGS1. Data represent three independent experiments.