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. 2015 Aug 12;1:15007. doi: 10.1038/npjbcancer.2015.7

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Copyright © 2015 Breast Cancer Research Foundation/Macmillan Publishers Limited

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Activin-A promotes anchorage-independent growth but not proliferation of breast cancer cells. (a) Bromodeoxyuridine (BrdU) incorporation assay following activin-A treatment of MCF7 (i) and MDA-MB-231 (iii) cells. Overexpression of INHBA in MCF7 cells (ii) and knockdown of INHBA by small hairpin RNA (shRNA) in MDA-MB-231 cells (iv). MCF-7 cells but not MDA-MB-231 cells show mild inhibition in proliferation on activin-A treatment. (b) Treatment of MCF-7 cells with activin-A results in reduced colony growth (i), but MCF-7 clones overexpressing activin-A show higher colony-forming ability (ii). Treatment of MDA-MB-231 cells with activin-A in soft agar does not show any effect (iii) and stable knockdown of activin-A in MDA-MB-231 cells results in a significant decrease in colony formation (iv).