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. 2015 May 27;1:15006. doi: 10.1038/npjbiofilms.2015.6

Figure 1.

Figure 1

Degradation of N-acyl homoserine lactones (AHLs) based on the function of acyl chain length in the floccular sludge. The degradation value is expressed as the signal half-life. A mixture of unsubstituted (a) and oxo-substituted (b) AHLs was added to the live (open circle) or the heat-inactivated (solid circle) sludge to a final concentration of 5 μM for each AHL. The sludge mixture was incubated at room temperature with constant shaking at 200 r.p.m. The pH of each sludge mixture (bulk liquid) was monitored throughout the study and ranged between pH 6.7 and 6.9. Residual AHLs were extracted and quantified using LC-MS/MS at different time points. The signal half-life for each AHL was estimated based on the zero-order or first-order kinetics. Error bars are defined as s.e.m. (n=3, biological replicates). Holm–Sidak multiple t-tests were performed (live versus heat-inactivated control for each AHL) and corrected P values (P<0.05 for all pairs of comparison) are reported.