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. 2015 May 27;1:15006. doi: 10.1038/npjbiofilms.2015.6

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Copyright © 2015 Nanyang Technological University/Macmillan Publishers Limited

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Distribution of isolates according to their capacity to produce and/or quench N-acyl homoserine lactones (AHLs). A total of 330 isolates, including 307 and 23 strains of bacterial and fungal origins, respectively, were cultured from the floccular sludge community during the bioreactor operation from weeks 40–44. All isolates were screened for AHL production by different bioassays and LC-MS/MS, as well as for their capability to quench 5 μM of AHLs with short (3OC6-HSL), medium (3OC8-HSL) and long (3OC12-HSL) acyl chains after 2 h incubation. Residual AHLs were quantified using the A. tumefaciens A136 agar-based spot bioassay.